CRISPR Gene Editing — Questions and answers

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What did Dr. Jennifer Doudna compare a single mistake in the "book of life" to?

Choices

  1. A misplaced comma or an incorrect word. ✓
  2. An entire chapter being rewritten.
  3. A very short book.
  4. A completely new book.

Answer: A misplaced comma or an incorrect word.

From page 1

Before CRISPR, what were the gene alteration tools compared to?

Choices

  1. A very sharp knife for surgery.
  2. A blunt instrument for delicate surgery. ✓
  3. A perfect pair of molecular scissors.
  4. A simple pencil and paper.

Answer: A blunt instrument for delicate surgery.

From page 2

What did researchers think the 'Cas proteins' were, based on Dr. Emmanuelle Charpentier's statement?

Choices

  1. The 'hands' that would interact with the genetic library to neutralize invaders. ✓
  2. The 'books' in the genetic library.
  3. The 'doors' to the genetic library.
  4. The 'keys' that locked up the invaders.

Answer: The 'hands' that would interact with the genetic library to neutralize invaders.

From page 4

What did Dr. Charpentier compare the discovery of tracrRNA to?

Choices

  1. Finding a new kind of food.
  2. Solving a very difficult math problem.
  3. Finding the missing piece of a molecular puzzle. ✓
  4. Learning to ride a bike.

Answer: Finding the missing piece of a molecular puzzle.

From page 5

What did Dr. Doudna call the single guide RNA (sgRNA) that her team engineered?

Choices

  1. A complicated maze.
  2. Our 'aha!' moment and a universal key. ✓
  3. A tiny microscope.
  4. A very long rope.

Answer: Our 'aha!' moment and a universal key.

From page 6

After Doudna and Charpentier's discovery, what important discussions did the new power of CRISPR-Cas9 open up?

Choices

  1. How to make the system even more complicated.
  2. How to make the system glow in the dark.
  3. Profound ethical questions about altering the human germline. ✓
  4. How to use it to play video games.

Answer: Profound ethical questions about altering the human germline.

From page 9